Cellular organization and cell organelles; lipid membranes; the cytoskeleton; and cell interactions signaling and cycle control. Growth factors and neurochemistry. Mechanical and electrical phenomena. Synaptic transmission. Sensory and motor neurophysiology.
Principles and methodologies involved in antigen preparation and presentation, antibody production and purification, isolation and immortalization of immune cells, immunohistochemical analyses and assays for complements and cytokines.
Rationale for making ethical decisions, review of existing guidelines, considerations of the use of adult and embryonic stem cells, ethical issues on animal research, conflict of interest and misconduct in research and business.
Product development and commercialization processes. Product life cycles, program management basics. Intellectual property. Regulatory affairs issues: GLP, product registration, GMP, documentation, validation, FDA inspections.
Preparation and isolation of antibodies, basics of cell culture, recombinant DNA techniques, techniques of protein production and engineering and examples of cloning. Basics of GLP and practical experience in applications of GLP.